incomplete adjuvant

Methods Spleen cells of Balb / c mouse immunized subcutaneously with cysticercus fluid ( mixture of Freund 's incomplete adjuvant and interferon ) were fused with myeloma cells SP2 / 0 , and the resulting hybridoma cells were selected and cloned .

方法运用自提的猪囊虫囊液与福氏不完全佐剂加干扰素混合免疫Balb/c小鼠，取小鼠脾淋巴细胞与SP2/0骨髓瘤细胞融合，反复筛选及克隆化；

Fourteen days later , the same antigen emulsified in in freund 's incomplete adjuvant were intraperitoneally . and three days after final immunization , Splenocytes from the immunized mice were fused with Sp2 / 0-Ag-14 myeloma cells . Keep it in the bloom of proliferation before cell fusion .

然后每隔14天后再用弗氏不完全佐剂与之混合，共免疫2次，末次加强免疫以100μg／只尾静脉注射，3d后取小鼠脾细胞与骨髓瘤细胞Sp2／0-Ag-14进行融合。

But incomplete adjuvant is adequate for subsequent injections .

但随后的注射则以使用不完全佐剂为宜。

With the chicken-derived anti-AIV IgG as the immune , the Freund 's complete adjuvant ( FCA ) vaccine and the Freund 's incomplete adjuvant ( FIA ) vaccine were prepared to incubated female SPF BALB / c mice of 8 weeks .

用以上提取的鸡抗AIVIgG作为免疫原，制备完弗氏全佐剂和不完全弗氏佐剂疫苗免疫8周龄雌性SPFBALB/c小鼠。

Ovalbumin group : Each rat was treated with autoantigen ovalbumin ( 200 μ g ) plus incomplete Freund adjuvant ;

卵白蛋白组：非自身抗原卵白蛋白加不完全福氏佐剂，卵白蛋白用量200μg/只。

After SDS-PAGE , the recovery of CP added the same volume of incomplete Freund Adjuvant emulsified completely , injected rabbits by subcutaneous injection .

PAGE电泳后，将表达的CP蛋白切胶回收，研磨成粉后加入等体积的福氏不完全佐剂，乳化制备成抗原。

Methods : The rabbits were classed into five groups , according to the different antigen injected and the incomplete Freud 's adjuvant .

方法：家兔按注射的抗原及佐剂的不同分组，皮下多点注射分别进行免疫。

METHODS : Mouse lymphangiomas in abdominal cavity were induced by incomplete Freund 's adjuvant , then disrupted and digested to obtain LEC , which were cultured in the flask or plate previously coated with rat-tail collagen .

方法：应用不完全弗氏佐剂诱导小鼠腹腔淋巴管瘤形成，消化法分离获得LEC，置于自制的鼠尾胶包被的培养瓶（板）中培养。

Established a rotating-shaking method for incomplete Freund 's . adjuvant ( IFA ) antigen 's preparation and compared this with IFA antigen which prepared by classic method when we produced highly finished tetanus antitoxin ( TAT ) .

建立了旋转振荡法配制弗氏不完全佐荆（IFA）抗原，并在精制破伤风抗毒素（TAT）生产中，与传统方法配制的IFA抗原进行比较。

Methods The emulsive mixture of TE kDNA and incomplete Freund ′ s adjuvant ( IFA ) was injected into normal BALB / c mice subcutaneously , intraperitoneally , intramuscularly or intravenously . These mice were immunized once every five days .

方法将马疫锥虫kDNA与不完全弗氏佐剂乳化混合，通过皮下、腹腔、肌肉及静脉等途径注射入正常BALB/C小鼠。